The GID 700TM is a sterile single-use disposable tissue canister used for harvesting, filtering, and transferring of autologous adipose tissue. The GID 700TM is CE mark certified, and is distributed in the United States by LifeCell Corporation as the "Revolve".
The GID SVF-1TM is a sterile single-use disposable tissue canister used for harvesting, filtering, separating, concentrating, and transferring autologous tissue components for reintroduction to the same patient during a single surgical procedure for repair, reconstruction, or replacement of integumentary or musculoskeletal tissues. The GID SVF-1TM is designed for easy and fast separation and concentration of the Stromal Vascular Fraction cells from tissue for tissue repair, reconstruction, or replacement in the same surgical procedure. The GID SVF-1TM is CE mark certified, and is not available in the United States.
The GID SystemTM includes thorough quality and process control systems designed to be simple and reliable; this allows optimized processing for maximal cell yields tuned to specific requirements and a real-time assessment for the physician or researcher.
GID's disposables and components are produced in QSR, ISO 13485, MDD 93/42/EEC certified manufacturing facilities. GID proprietary reagent blends produced by certified manufacturer with GMP production capability. Because the GID SystemTM is modular and scalable, disposable and reagent costs are optimized for the required volume; the GID SystemTM also uses off-the-shelf laboratory equipment wherever possible, thus minimizing the cost associated with custom capital equipment.
Methods of separating and concentrating the Stromal Vascular Fraction (SVF) components from adipose tissue are well-known and widely published (see Literature Citations: 2,7,9,10,11). The basic form of all methods is to harvest adipose tissue in the form of lipoaspirate, use a collagenase enzyme to disaggregate and separate the tissue components, then concentrate the tissue components by density gradation using a centrifuge. Methods vary in the presence or lack of washing steps, amount and concentration of collagenase used, time of the enzyme reaction, temperature maintenance, and technique for removal of waste tissues. Methods also vary widely by type of equipment used such as sterile collection canisters, test tubes, filters, tubing sets, Falcon tubes, and other general laboratory equipment applicable to tissue processing.
Using the GID system, the entire process occurs within a single disposable container. The system provides fast, repeatable, predictable and cost effective results, and includes real-time quality assays of cell count and viability.
1. Calabrese, C., et al., Breast reconstruction after nipple/areola-sparing mastectomy using cell-enhanced fat grafting. Ecancermedicalscience, 2009. 3: p. 116.
2. Cervelli, V., et al., Application of enhanced stromal vascular fraction and fat grafting mixed with PRP in post-traumatic lower extremity ulcers. Stem Cell Res, 2011. 6(2): p. 103-11.
3. Kamakura, T. and K. Ito, Autologous cell-enriched fat grafting for breast augmentation. Aesthetic Plast Surg, 2011. 35(6): p. 1022-30.
4. Levi, B. and M.T. Longaker, Concise review: adipose-derived stromal cells for skeletal regenerative medicine. Stem Cells, 2011. 29(4): p. 576-82.
5. Moseley, T.A., M. Zhu, and M.H. Hedrick, Adipose-derived stem and progenitor cells as fillers in plastic and reconstructive surgery. Plast Reconstr Surg, 2006. 118(3 Suppl): p. 121S-128S.
6. Perez-Cano, R., et al., Prospective trial of adipose-derived regenerative cell (ADRC)-enriched fat grafting for partial mastectomy defects: the RESTORE-2 trial. Eur J Surg Oncol, 2012. 38(5): p. 382-9.
7. Rodriguez, J.P., et al., Autologous stromal vascular fraction therapy for rheumatoid arthritis: rationale and clinical safety. Int Arch Med, 2012. 5: p. 5.
8. Yoshimura, K., et al., Cell-assisted lipotransfer for cosmetic breast augmentation: supportive use of adipose-derived stem/stromal cells. Aesthetic Plast Surg, 2008. 32(1): p. 48-55; discussion 56-7.
9. Yoshimura, K., et al., Cell-assisted lipotransfer for facial lipoatrophy: efficacy of clinical use of adipose-derived stem cells. Dermatol Surg, 2008. 34(9): p. 1178-85.
10. Astori, G., et al., "In vitro" and multicolor phenotypic characterization of cell subpopulations identified in fresh human adipose tissue stromal vascular fraction and in the derived mesenchymal stem cells. J Transl Med, 2007. 5: p. 55.
11. Yoshimura, K., et al., Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates. J Cell Physiol, 2006. 208(1): p. 64-76